Figure 3.
Figure 3. Stimulation of neutrophil NADPH oxidative bursts using Abs attached to GST-MSP119-coated microtiter plates. Chemiluminescence (CL; arbitary units) was induced by (A) anti-MSP119 human IgA, or (B) antihuman MSP119 human IgG1 at (▪) 1 × 10-6 M, (▴) 5 × 10-7 M, (•) 1 × 10-7 M, and (□) 5 × 10-8 M. Isotype controls (○) IgA1-NIP or IgG1-NIP, or (♦) MSP119 specific mouse IgG2b at 1 × 10-6 M. (C) Diabody or Ab at 1 × 10-6 M; (▪), FcγRI × MSP119; (•), IgG1-MSP119; (♦), IgG2b-MSP119; (□), IgG1 isotype control; ▵, no Ab or diabody. In each case the experiments were performed 4 times with neutrophils from different donors with similar results.

Stimulation of neutrophil NADPH oxidative bursts using Abs attached to GST-MSP119-coated microtiter plates. Chemiluminescence (CL; arbitary units) was induced by (A) anti-MSP119 human IgA, or (B) antihuman MSP119 human IgG1 at (▪) 1 × 10-6 M, (▴) 5 × 10-7 M, (•) 1 × 10-7 M, and (□) 5 × 10-8 M. Isotype controls (○) IgA1-NIP or IgG1-NIP, or (♦) MSP119 specific mouse IgG2b at 1 × 10-6 M. (C) Diabody or Ab at 1 × 10-6 M; (▪), FcγRI × MSP119; (•), IgG1-MSP119; (♦), IgG2b-MSP119; (□), IgG1 isotype control; ▵, no Ab or diabody. In each case the experiments were performed 4 times with neutrophils from different donors with similar results.

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