Isolation and purification of the CCE-25–c-Kit^neg cells. Representative forward and side scatter of mouse bone marrow (A). Forward scatter (FSC) and side scatter (SSC) profile of CCE-25–c-Kit^neg cells isolated by elutriation according to the procedures outlined in “Materials and methods” (B). FACS analysis of CCE-25 cells incubated with lineage- and c-Kit–specific antibodies (Lin: B220, CD3ϵ, TER-119, Gr-1) (C). The CCE-25–Lin^negc-Kit^neg cells were gated and examined for the expression of Sca-1 (D), CD34 (E), CD38 (F), CD43 (G), CD45 (H), and Thy 1.2 (I). CCE-25–c-Kit^neg cells (J) were sorted for Sca-1^pos and Sca-1^neg (K) populations and their purity was verified by postsort reanalysis (L-M). Isotype-matched control background staining was subtracted from the percentages shown. These results are representative of 2 experiments.