Figure 3.
SLAP does not interfere with intrinsic erythroid differentiation. (A) Quantitative determination of hemoglobin levels in representative BCL-2 and BCL-2/SLAP clones at days 1, 2, and 3 after differentiation induction in the absence of Epo. Normalized values (hemoglobin level per 106 live cells) are plotted. (B) Cytocentrifugation analysis of representative BCL-2 and BCL-2/SLAP erythroblast clones maintained in absence of Epo for 3 days. Cells were stained with neutral benzidine (stains hemoglobin in brown) and histologic dyes (Diff-Quik). Original magnification × 60.