Following T-cell-depleted D^-/R⁺ alloSCT, a primary HCMV-specific CD8⁺ T-cell response developed in recipient 05 within 12 months. An HCMV pp65_495-503-specific CD8⁺ T-cell clone (clone 5.2) was obtained from the HCMV-seropositive recipient at 21 months after T-cell-depleted alloSCT. The clone was not detected in the donor or in recipient 05 prior to transplantation. Following an episode of HCMV retinitis at 5 months, the clone was first detected in recipient 05 at 12 months after alloSCT. The retinitis resolved following ganciclovir therapy, and subsequent weekly blood tests for HCMV DNA were negative.