C/EBPα 30-kDa and BRM2 mutant proteins cannot induce the downstream granulocytic genes C/EBPϵ and the G-CSF receptor. (A) The figure demonstrates Northern blot analysis of C/EBPϵ and G-CSF receptor RNA in stably transfected K562 lines following induction with β-estradiol up to 48 hours. To control for RNA integrity and amount, the same blot was probed with c-Myc and GAPDH as shown in Figure 5A. (B) The procedure was the same as for panel A, except this was an independent experiment with different Northern blots. The same blots were probed with c-Myc and GAPDH as shown in Figure 5B. The blots for the 42-kDa C/EBPα-ER fusion in panels A and B are 2 completely independent experiments from 2 different inductions of transfected K562 cells.