CD4⁺ CD25⁺ T cells prevent CD8⁺-mediated skin inflammation. Ears from the same Ii^°/° recipients as those in Figure 3 were harvested 96 hours after DNFB challenge. Cryostat sections of ears were either stained with hematoxylin/phloxin/safran (A-D) or with an anti-CD8 mAb (red) and counter-stained with hematoxylin (E-G). Original magnification, × 40. No CD8-specific staining or local inflammatory reaction was detected in sections of ears from nonsensitized animals (not shown).