Blockade of Hsp27 by AS-Hsp27 restores sensitivity to Dex in MM.1R cells. (A) Functional specificity of AS-Hsp27 was determined by subjecting protein lysates from AS-Hsp27-transfected or control vector-transfected cells to immunoblot analysis with anti-Hsp27 (upper panel) or anti-Hsp70 antibodies (lower panel). (B) MM.1R cells were transiently transfected with cDNA construct containing GFP-tagged AS-Hsp27 (▦) or with GFP-empty vector (▪). Following transfections, GFP⁺ cells were selected by flow cytometry; treated with Dex (5 μM) for 24, 48, or 72 hours, and analyzed for cell viability by MTT assay (P = .05, as determined by one-sided Wilcoxon rank sum test). Error bars represent standard deviation.