Figure 3.
![Figure 3. TPO does not enhance Hoxa9 expression. (A) UT-7/TPO cells were cultured with 10 ng/mL or 100 ng/mL TPO for 24 hours, total RNA was prepared, and real-time RT-PCR was performed to quantify HOXA9 level. The bars represent the average ± SD of several independent experiments (n = 3, TPO 10 ng/mL; n = 5, TPO 100 ng/mL). (B) Whole bone marrow cells were prepared from control C57BL6 and tpo[–/–] mice. The cells were pooled (experiment 1: n = 4, C57BL6, n = 4, tpo[–/–] mice; experiment 2: n = 4, C57BL6, n = 5, tpo[–/–] mice) and Sca-1+/c-kit+/Gr-1– cells were collected for real-time PCR analysis. Each column represents the average Hoxa9 level normalized to GAPDH internal control ± SD of 2 experiments (▪, C57/BL6; ▨, tpo[–/–] mice.)](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/9/10.1182_blood-2003-03-0944/6/h82135169003.jpeg?Expires=1735527640&Signature=EzYB7n2sS0O6ujpMeCVXcdPbd66SvFPu64SKN2zmLj9~UpaMGJ2frga2X2-Ot7O7toghArmiYQ~orRyU8QLzeyzgFkYohCp67LPt61g9kEtdnKXa-AFW2eCKR1TXlBBhtp2tNVdZqDsvPYB~EYwHoBG1XGR8ATCCjlKqeo70rQ7qQXG057OP~DF0j1Th1jW03z0ulc6lxdNU5pgwPHz3aG9rwvjRYIHpbaZZ6QGHQPhC9kb2~M4JnlkM7rVbn0pTMX3Hbar1IzzHGC7Mtv5cf3J4Felycw~gTQiO84tluHx2yRlfUCnAU3Dl38mmcNoiR3hQMFWORCB9gY78473o2w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TPO does not enhanceHoxa9expression. (A) UT-7/TPO cells were cultured with 10 ng/mL or 100 ng/mL TPO for 24 hours, total RNA was prepared, and real-time RT-PCR was performed to quantify HOXA9 level. The bars represent the average ± SD of several independent experiments (n = 3, TPO 10 ng/mL; n = 5, TPO 100 ng/mL). (B) Whole bone marrow cells were prepared from control C57BL6 and tpo[–/–] mice. The cells were pooled (experiment 1: n = 4, C57BL6, n = 4, tpo[–/–] mice; experiment 2: n = 4, C57BL6, n = 5, tpo[–/–] mice) and Sca-1+/c-kit+/Gr-1– cells were collected for real-time PCR analysis. Each column represents the average Hoxa9 level normalized to GAPDH internal control ± SD of 2 experiments (▪, C57/BL6; ▨, tpo[–/–] mice.)
