Figure 3.
Spleen contains predominantly human B cells and only rare human DCs. Immunofluorescence and confocal analysis of frozen tissue sections of spleen harvested from different cohorts of mice (A, mouse that received a transplant of 6 × 105 fetal liver CD34+ HPCs; and B, mouse that received a transplant of 5 × 106 adult peripheral blood CD34+ HPCs. (A) Triple staining with anti–human CD11c-FITC (green), anti–human CD45-TxR (red) and anti–human HLA-DR-Cy5 (blue). Single fluorescence and overlay at × 40 magnification. Note only few cells with the phenotype of mDCs. (B) Double staining with anti–human IgD-FITC (green) and anti–human CD45 TxR (red). Single fluorescence and overlay at × 63 magnification.
