Plasmacytoid and myeloid DCs in the bone marrow of NOD/SCID mice engrafted with human CD34⁺ HPC. (A) Frequency of pDCs and mDCs in the total bone marrow (n = 18) as determined by the frequency of lineage negative, HLA-DR⁺ cells with reciprocal expression of CD123 and CD11c, respectively. (B) Flow cytometry plots of mDCs and pDCs analysis. (C) Low migration of human DCs into spleen is confirmed by the flow cytometry analysis of single cell suspensions (n = 13, all subsets and tissue localization per each of the 13 mice that received transplants of CD34⁺ HPCs from various sources).