ASO-PCR analysis using DNA 500 chip in case 472. U indicates upper marker; L, lower marker; P, presentation sample; and R, relapse sample. (A) IgH (V_H3) gene rearrangement from diagnostic sample using a combination of V_H3 family-specific primer and clone-specific primer based on sequence identified at relapse. The peaks indicated by an arrow show the PCR product. (B) ASO-PCR result of IgH-V_H3 gene rearrangement from relapse tumor sample as a positive control (280 bp). Distinctive sharp peak of the ASO-PCR product is indicated by arrows. The PCR primers were the same as in panel A. (C) The IgH (V_H1) gene rearrangement from the diagnostic sample using combination of V_H1 family-specific primer and clone-specific primer based on sequence identified at relapse. The peak indicated by an arrow shows the PCR product. (D) ASO-PCR result of IgH-V_H1 gene rearrangement from relapse tumor sample as a positive control (271 bp). Distinctive sharp peak of the ASO-PCR product is indicated by an arrow. The PCR primers were the same as in panel C. (E) ASO-TCRδ gene rearrangement from diagnostic sample using combination of relapse clone-specific primer and Dδ3 primer. The peak indicated by an arrow shows the PCR product. (F) ASO-PCR result of TCRδ gene rearrangement from relapse tumor sample as a positive control (100 bp). The distinctive sharp peak of the ASO-PCR product is indicated by an arrow. The PCR primers were the same as in panel E.