T1 and T2 growth. (A) T1 and T2 growth curve. T cells were stimulated with anti-CD3/anti-CD28–coated beads on day 0 and were cultured in type 1 or type 2 media conditions for 6 days. (B) Surface flow cytometry plot of CD4⁺ and CD8+ T1 and T2 cells. T1 and T2 cells were stained with anti-CD4 PE and anti-CD8 FITC mAbs on day 6 of culture. The percentages of CD4⁺ and CD8+ T cells are indicated in the upper left and lower right quadrants, respectively.