Determination of the acquisition of alloreactive T cells in T1 and T2 recipients. (A, B) T1 and T2 populations were generated from either syngeneic CB6F1 or allogeneic Ly5.1 B6 donor mice and administered intravenously to lethally irradiated CB6F1 mice. On day 5 after BMT, splenic cells were isolated and stimulated in vitro with either syngeneic (B6) or allogeneic (CB6F1) DCs (10:1 T cell/DC ratio). After 24 hours of stimulation, the supernatants were harvested and tested for cytokine content (IFN-γ, IL-2, IL-4, IL-5, and IL-10) using ELISA. Results, which represent the only cytokines detected, are shown as mean ± SEM of values obtained (n = 5 per treatment group).