Expression of truncated ABIN-2 inhibits Ang1^*-stimulated endothelial survival. (A) HUVECs were transfected with GFP and control vector (CV) or with vector encoding wild-type ABIN-2 (ABIN-2-WT) or ABIN-2 lacking 85 amino acids of the carboxy-terminus (ABIN-2-ΔC), as indicated. Cell survival of transfected cells was determined as described in “Study design.” The lower part of panel A depicts a Western blot showing expression of wild-type ABIN-2 (ABIN-2-WT) (arrow) and ABIN-2-ΔC (arrowhead) in HUVECs transfected with CV, ABIN-2-WT, or ABIN-2-ΔC as indicated. The presence of expressed forms of ABIN-2 was detected by probing for the N-terminal FLAG-epitope tag. Relative mobility of molecular mass markers is shown in kilodaltons. (B) HUVECs were transfected with GFP and CV or truncated ABIN-2 as indicated. Twenty-four hours after transfection, cells were subjected to growth factor withdrawal in the absence or presence of 400 ng/mL Ang1^* and cell survival determined at 18 hours as detailed in “Study design.” Data are presented as means and SEM for 5 independent experiments (A) or 3 independent experiments (B). ^*P < .01 vs CV.