Figure 2.
Figure 2. Bcl2 gain of function glutamate but not phosphorylation-deficient alanine mutants potently reduce intracellular ROS and inhibit oxidative stress-induced apoptosis. (A) Cells expressing WT and A- or E-Bcl2 mutants were incubated with the oxidation-sensitive dichlorodihydrofluorescein diacetate (H2DCFDA) at 37°C for 30 minutes, and intracellular ROS levels were analyzed by flow cytometry as described.8,15 (B) Cells expressing WT and A- or E-Bcl2 mutants were treated with various concentrations of H2O2 for 24 hours as indicated. Cell viability was determined by analyzing annexin-V binding on FACS. Data represent the mean ± SD of 3 determinations.

Bcl2 gain of function glutamate but not phosphorylation-deficient alanine mutants potently reduce intracellular ROS and inhibit oxidative stress-induced apoptosis. (A) Cells expressing WT and A- or E-Bcl2 mutants were incubated with the oxidation-sensitive dichlorodihydrofluorescein diacetate (H2DCFDA) at 37°C for 30 minutes, and intracellular ROS levels were analyzed by flow cytometry as described.8,15  (B) Cells expressing WT and A- or E-Bcl2 mutants were treated with various concentrations of H2O2 for 24 hours as indicated. Cell viability was determined by analyzing annexin-V binding on FACS. Data represent the mean ± SD of 3 determinations.

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