Phosphomimetic Bcl2 enhances expression of Cu/Zn-superoxide dismutase (SOD1) and catalase, and more potently inhibits 3-AT or 2-ME–induced cell death. (A) Cells expressing WT, A-, or E-Bcl2 mutants were lysed in detergent buffer. Expression levels of SOD1 and catalase were determined by Western blotting using SOD1 or catalase antibody, respectively. (B) Cells were treated with 3-AT (10 mM) or 2-ME (3 μM) for 72 hours. Cell viability was determined by analyzing annexin-V binding on FACS as described in Figure 1C. The data represent the mean ± SD of 3 determinations.