Figure 4.
![Figure 4. IFN-γ production by Kb-CD28-ζ and Kb-CD28[L→G]-ζ RMTCs. GFP-sorted CD8+ RMTCs were stimulated 7 days after transduction in the presence of splenocyte feeders on plates coated with 5 μg/mL AF6-88.5 anti-Kb, with conA, or cultured in the absence of stimulation. Stimulation-induced IFN-γ production was measured by Bioplex assay using anti-IFN-γ-coated beads. Data points are means of triplicate samples. Error bars show ± 1 SD; *, less than 1 ng/mL.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/13/10.1182_blood-2003-04-1255/6/h82435385004.jpeg?Expires=1735544834&Signature=oZB3EqjQf7yhcfDGdRMt~vTid7nbIrHYDxNyGRgZ~7VBBQlQdi~MdC279Rs-ZSFdhVsLn9onCaIhuwLvbXWDgektbWwm1SswgxzItLUpYfC7Y0pVUf~5O45yOvF6t3Esbn~LxkE5VZbitGCX3eyA2mbER0aLgDWjf0od4dkmdBKmjIrwbr50-AAbTrDVRCJNx7PU4d~KBo-dj4oashgB1xzszWJGMPDt86vXzgV6z5Fr8eHJboXWIKa46nwuTSkkAwpsNhwDndFNj9yA95wI8kkdNhTl-GRhptKPz1SET1PC~3MKI1UpCo9XivKHWn9KfJyXoJZdPBuEWR8~DJn6Og__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IFN-γ production by Kb-CD28-ζ and Kb-CD28[L→G]-ζ RMTCs. GFP-sorted CD8+ RMTCs were stimulated 7 days after transduction in the presence of splenocyte feeders on plates coated with 5 μg/mL AF6-88.5 anti-Kb, with conA, or cultured in the absence of stimulation. Stimulation-induced IFN-γ production was measured by Bioplex assay using anti-IFN-γ-coated beads. Data points are means of triplicate samples. Error bars show ± 1 SD; *, less than 1 ng/mL.
