Representative Western blot analysis of cloned gene protein products using anti-GST monoclonal antibody and sera from 2 AA patients. GST fusion proteins were induced by IPTG, separated by 10% SDS-PAGE, and transferred to PVDF membranes. Triplicates of membranes were prepared and probed with mouse anti-GST monoclonal antibody, patient 1AA serum, or patient 3AA serum. Immunoreactive proteins were detected with horseradish peroxidase-conjugated goat antimouse or human IgG secondary antibody.