Sera from AA patients show different positive patterns for recombinant GST-full-length kinectin protein on Western blot analysis. Equal amounts of bacterial lysates expressing GST (lane 1) and GST-full-length kinectin fusion protein (lane 2) were separated by 9% SDS-PAGE, transferred to PVDF filters, and probed with mouse anti-GST monoclonal antibody, antikinectin monoclonal antibody, AA patient sera, or normal sera. Immunoreactive proteins were detected with horseradish peroxidase-conjugated goat antimouse or antihuman IgG secondary antibody. Arrows indicate the immunoreactive GST-kinectin fusion proteins.