Partial epitope mapping of kinectin IgG autoantibody using GST-kinectin fusion proteins. We made a series of GST-partial kinectin fusion proteins using PCR-based subcloning. Equal amounts of lysates expressing various different fusion proteins were separated by 9% SDS-PAGE, and Western blot analysis was performed. Blots were probed with mouse anti-GST monoclonal antibody (A), antikinectin monoclonal antibody (B), patient 1AA serum (C), or patient 7AA serum (D). Immunoreactive proteins were detected as described in previous figures. (E) Results of partial epitope mapping of kinectin IgG autoantibody in 6 AA patients' sera are shown. Regions of the kinectin sequence shaded with identical patterns represent areas that are immunoreactive with multiple AA patient sera.