Standard cytotoxicity assay. We raised CTLs against kinectin-derived peptides 794S (A) and 645F (B) from healthy donors. Peptide-pulsed T2 cells were incubated with differing E/T ratios in a 4-hour cytotoxicity assay. (A) CTLs raised against the 794S peptide specifically lyse 794S pulsed (▪) but not T2 cells pulsed with an irrelevant peptide (▴, F271 from MAGE-3). (B) Similarly, CTLs generated against the 945F peptide recognize only T2 cells pulsed with 645F (□) but not control T2 cells (▵, pulsed with F271; ⋄, unpulsed).