IL-10 inhibits cytokines and gene induction by ISS-stimulated PBMCs. Freshly isolated PBMCs (2 × 10⁶ cells/mL) were stimulated with ISS (20 μg/mL) for 24 hours alone (black bars) or with IL-10 (10 ng/mL) (dotted bars) or anti-human IL-10R (2.5 μg/mL) (hatched bars). (A) Cytokine production was evaluated with the use of immunoassay. (B) Level of gene expression was assessed by quantitative PCR analysis (Taqman). Results shown are representative of more than 20 donors for cytokine production and of 8 donors for gene expression analysis.