Chemotaxis of human dendritic cells in response to EDN and mEAR2. (A) Specific abrogation of EDN-induced chemotaxis CD34⁺ cell–derived iDCs by RI. RI was mixed with EDN (1000 ng/mL) or SDF-1α (100 ng/mL) at concentrations as specified and the mixtures were added to the lower wells of chemotaxis chambers. (B) DC chemotactic activities of several members of the RNase A superfamily. Chemotaxis of iDCs is shown as the average cell migration (mean ± SD) of triplicate wells. hPR and bPR indicate human and bovine pancreatic RNase, respectively; hANG, human angiogenin; and mEAR2, murine eosinophil–associated RNase 2.