EDN and mEAR2 as chemoattractants for mouse DCs. The migration of iDCs and mDCs generated from mouse bone marrow HPCs in response to EDN or mEAR2 was investigated by chemotaxis assay and the results are shown as the average cell migration (mean ± SD) in triplicate wells. (A) Dose-responses. (B) Blockade of EDN- or mEAR2-induced migration of iDCs by the presence of placental RI at 1000 U/mL. (C) Inhibition of EDN-induced mDC migration by PTX. Mature DCs were preincubated at 37°C for 30 minutes in the absence (PTX^-) and presence (PTX+) of PTX (200 ng/mL) prior to chemotaxis assay. In panels B and C, EDN or mEAR2 was used at 1000 ng/mL.