Effects of R115777 on the levels of P-Akt/Akt, P-STAT3/STAT3, P-Erk1/2/Erk1/2, and β-actin in PBMCs from patients with MDS. PBMCs were processed for Western immunoblotting and probed with antibodies against phosphorylated and total Akt, STAT3, and Erk1/2, as described in “Patients, materials and methods.” β-Actin was used as loading control. BL, D7, and D8 indicate baseline and days 7 and 8 after R115777 treatment initiation.