Induction of IL-1β production, but not IL-12, IL-6, or TNF-α, by Fas ligation. (A) Cytokine production by DCs from normal C57BL/6J mice or lpr mice was measured using ELISA after DCs were stimulated with 1 μg/mL Jo-2 (Jo-2), isotype antibody (Iso), and 5 μg/mL rmFasL in the presence of 10 μg/mL anti-6 × histidine antibody (FH) or 0.2 μg/mL LPS (LPS) for 24 hours. Unstimulated DCs were used as control (con). (B) Production of IL-1β by immature DCs after stimulation with 1 μg/mL Jo-2 for various lengths of time. (C) IL-1β production by immature DCs after stimulation with various concentrations of Jo-2 (0.1 μg/mL-10 μg/mL). (D) IL-1β mRNA expression in DCs stimulated with medium alone (C), Jo-2 (J), isotype antibody (I) or LPS (L) at indicated time points. Results are representative of 3 independent experiments. (E) Effect of cycloheximide (CHX) on Jo-2-induced IL-1β secretion. DCs were stimulated with Jo-2, isotype antibody or LPS, for 24 hours in the presence or absence of 0.5 μg/mL CHX, and then IL-1β levels in supernatant were measured by ELISA.