DC maturation is induced by Fas ligation and recombinant mouse IL-1β (A) DCs were treated with Jo-2, isotype antibody (isotype 1), or recombinant mouse IL-1β for 24 hours or were pretreated with anti-mIL-1β antibody or isotype antibody (isotype 2) for 30 minutes, then treated with Jo-2 or isotype 1 for 24 hours. DCs were then stained with specific antibodies against MHC class II, CD86, or CD40 and were analyzed by flow cytometry. Plots are labeled with the percentages of mature DC. (B) Proliferation of allogeneic T cells induced by Jo-2-stimulated DCs. Data represent mean ± SD.