Reduction of IL-7Rα (CD127) expression on naive T cells from cGVHD^- patients. (A) FACS analysis of IL-7Rα⁺ naive T-cell frequencies of AHSCT patients and control group. CD127⁺ lymphocytes were quantified in CD3⁺, CD45RA⁺, CD27⁺ naive T cells by multiparameter flow cytometry. A minimum of 1 × 10⁵ events in the live cell gate, as defined by forward and side scatter, was accumulated for each sample. A “percentile” analysis was performed for the control and AHSCT GVHD^- patient groups in order to illustrate the data. The top and bottom horizontal lines represent the 90% and 10% percentile values, respectively, whereas the top and bottom edges of the rectangle represent the 75% and 25% percentile, respectively. The horizontal line located within the rectangle defines the 50% percentile. (B) Mean fluorescence intensity (MFI) of CD127-expressing CD3⁺, CD45RA⁺, CD27⁺ naive T cells of AHSCT patients and control groups. Again, “percentile” analysis, identical to panel A, was performed to represent the data for the control and AHSCT GVHD^- patient groups. (C) Representation of the mean fluorescence intensity (MFI) of CD127^- and Bcl-2 expression on naive T cells. The vertical and horizontal solid lines represent the average IL-7Rα and Bcl-2 MFI of the control group. Empty triangles (▵) represent healthy adults, whereas empty circles (▴) correspond to cGVHD^- patients. Representative example of Bcl-2 (top left panel) and CD127 (bottom right panel) are shown for control who did not undergo transplantation (filled area) and AHSCT patient (empty area).