Cross-presentation of MP is dependent on active transport into the ER by TAP. Immature HLA A^*0201⁺ DCs were infected with Vac-ctr or Vac-ICP47 for 4 hours at an MOI of 2. The Vac-ctr- or Vac-ICP47-infected DCs were cocultured about 12 hours with apoptotic or necrotic monocytes expressing MP or MP peptide and a maturation stimulus. The left axis shows cross-presentation; the right, peptide presentation. The ratio of dead cells to DC was 2:1 and MP peptide 100 nM. All groups of DCs were fixed by glutaraldehyde, cocultured with Flu16 and responses assessed by IFN-γ ELISPOT assay. The DC/T cell ratio was 4:1. One representative experiment of 4 is shown.