Figure 3.
Figure 3. LANA is not an upstream activator of JNK. (A) Western blot for total and phospho-JNK on protein extracts (20 μg/lane) from 293 TetOff-EGFP-LANA cells were maintained with or without doxycycline (dox, 1 μg/mL, to suppress EGFP-LANA expression). Top panels: Western blot for phospho-JNK (P-JNK) and total JNK; middle panel: Western blot for actin to confirm equivalent protein loading; bottom panel: Western blot for EGFP-LANA. (B) 293 TetOff-EGFP-LANA cells were maintained with or without dox (1 μg/mL) and treated with TPA (20 ng/mL) or vehicle control for 20 minutes. Total cellular protein (250 μg) was subjected to a JNK in vitro kinase assay. Bottom panel: Western blot for total JNK on immunoprecipitated protein; top panel: Western blot for phospho-c-Jun (P-Jun).

LANA is not an upstream activator of JNK. (A) Western blot for total and phospho-JNK on protein extracts (20 μg/lane) from 293 TetOff-EGFP-LANA cells were maintained with or without doxycycline (dox, 1 μg/mL, to suppress EGFP-LANA expression). Top panels: Western blot for phospho-JNK (P-JNK) and total JNK; middle panel: Western blot for actin to confirm equivalent protein loading; bottom panel: Western blot for EGFP-LANA. (B) 293 TetOff-EGFP-LANA cells were maintained with or without dox (1 μg/mL) and treated with TPA (20 ng/mL) or vehicle control for 20 minutes. Total cellular protein (250 μg) was subjected to a JNK in vitro kinase assay. Bottom panel: Western blot for total JNK on immunoprecipitated protein; top panel: Western blot for phospho-c-Jun (P-Jun).

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