Figure 4.
Detection of retrovirally marked hematopoietic clones contributing to engraftment in NOD/SCID mice compared with autologous engraftment. LAM-PCR was used to identify individual clones contributing to hematopoiesis after transplantation. Peripheral blood samples of baboon A00066 at 6 weeks and at 6 months after transplantation and NOD/SCID marrow samples were analyzed. Note that engraftment is oligoclonal in all NOD/SCID mice. *Bands that were subjected to insertion site sequencing and had unique proviral integration sites. Bands 1, 5, 8, and 10 were sequenced and showed common integration sites between baboon and NOD/SCID repopulating cells.