Functional analysis of peptide-specific T cells contained in polyclonal OFA-iLRP-specific CTL cultures. T2 cells were separately pulsed with different peptides deduced from the OFA-iLRP protein (iLRP1-4) or with a control peptide (FluM1_58-66) and used as target cells in a 4-hour ⁵¹Chromium release assay (A). Killing activity of polyclonal OFA-iLRP-specific CTLs against T2 cells pulsed with different amounts of peptide iLRP1 and iLRP2 (B). Detection of iLR-peptide-specific T cells in polyclonal T-cell cultures using the IFN-γ and IL-10 ELISPOT assay (C). iLRP1 versus no peptide, P < .001; iLRP2 versus no peptide, P < .001; iLRP2 versus iLRP1, P < .005.