Effect of increasing RBC chimerism on the decay kinetics of biotinylated peripheral RBCs. (A) Biotinylation curves for representative mice that received transplants and control mice. y-axis = percentage of biotinylated RBCs remaining. x-axis = time (days) after biotinylation. Average survival curves for mice with 0% (sickle mice that did not receive transplants, •), 17% to 43% (▴), 61% to 76% (▴), 87% to 91% (▵), and 100% RBC chimerism (▪) are shown. (B) The relationship between the time at which 50% of the biotinylation signal had disappeared (biotinylation₅₀ time, y-axis) and the percentage of donor Hb in the peripheral blood (x-axis) is shown for sickle (0% donor Hb) and eGFP (100% donor Hb) controls (▴) and for the mice that received transplants (•).