HSCs give rise to circulating hematopoietic cells and functional ECs. The liver of recipients that received transplants of 500 GFP⁺ KSL cells was evaluated. (A) GFP⁺ KSL cell–derived blood cells within a longitudinal section of a hepatic vessel (lumen (L) outlined, FITC channel only). (B) Merged image showing donor, GFP⁺ (green), DAPI⁺ (blue) nucleated blood cells coexpressing CD45 (red) and a single host-derived GFP^–, CD45⁺ blood cell (arrow). (C) A portal vein with a donor DAPI⁺, GFP⁺, CD45^– EC (arrowhead) near a donor DAPI⁺, GFP⁺, CD45⁺ blood cell (arrow). (D) Low-density lipoprotein (Dil-Ac-LDL, red) uptake in GFP⁺ ECs. Insert shows higher magnification of a single GFP⁺, Dil-Ac-LDL cell (arrow head). (E) A GFP⁺, CD45^–, DAPI⁺ EC (arrowhead) within a portal vein from a 500 GFP⁺ KSL recipient 2 weeks after transplantation not injected with ac-LDL. Scale bars: A-B, 10 μm; C, 5 μm; and D-E, 20μm; original magnification, × 60 (D-E inserts).