Transplantation of a single HSC produces multilineage hematopoiesis in the peripheral blood of recipient mice. Top panels: Isolation of single GFP⁺ KSL cells. (Primary and secondary cell sorting gates are shown). Middle panel: Donor-derived cells in the peripheral blood of a recipient of a single GFP⁺ KSL cell. Lower panels: GFP⁺ peripheral blood myeloid cells (GR-1) and B cells (B220) derived from a single KSL cell.