VSMCs do not respond to IFN-γ stimulation in coculture. (A) Lysates were prepared from MACS-separated VSMCs on coculture and were stimulated with IFN-γ. Proteins were separated on SDS-PAGE and analyzed by Western blotting with anti-pSer727 Stat1 rabbit polyclonal antibody. VSMCs in monoculture with and without stimulation are included. As a control for gel loading, anti-Stat1 antibody was used. (B) DNA binding activity of nuclear extracts prepared from VSMCs treated with or without IFN-γ from monoculture and coculture was analyzed by EMSA.