Analysis of changes in gene expression observed in unstimulated and stimulated CD4⁺ T cells isolated from UCB and AB. The changes in gene expression were assessed using the HG-U133A&B microarray. One-way hierarchical clustering (A) was performed on the trimmed data set using GeneSpring v5.1 following per gene normalizations of the signal. Each gene or probe set was normalized to the median of its signal across all arrays. The gene tree was constructed using the 2521 probe sets which met the inclusion criteria^* in at least 1 of the 3 time points. Red and green indicate increased and decreased expression relative to the median, respectively. The Pearson correlation with a minimum distance of 0.001 was used in constructing the gene tree. (B) A Venn diagram depicts the total number of probe sets meeting inclusion criteria^* at each time point. ^*At least one present (P) call and FC of at least 2 and change not equal to no change (NC).