American Society of Hematology

Figure 2.

$Figure 2. Characterization of LDTI membranes isolated from K562 and Daudi cells. K562 and Daudi cells were lysed in Triton X-100 and subjected to sucrose gradient centrifugation. Aliquots of fractions collected from the top of the gradient were analyzed by Western blotting with the use of antibodies against Lyn, flotillin-1, early endosomal antigen 1(EEA1), and MHC class II (α chain) molecules, and by dot blot for GM1 content with the use of peroxidase-coupled cholera toxin B subunits. Threefold lower amounts of the 4 bottom gradient fractions were loaded on SDS-PAGE.$

Characterization of LDTI membranes isolated from K562 and Daudi cells. K562 and Daudi cells were lysed in Triton X-100 and subjected to sucrose gradient centrifugation. Aliquots of fractions collected from the top of the gradient were analyzed by Western blotting with the use of antibodies against Lyn, flotillin-1, early endosomal antigen 1(EEA1), and MHC class II (α chain) molecules, and by dot blot for G_M1 content with the use of peroxidase-coupled cholera toxin B subunits. Threefold lower amounts of the 4 bottom gradient fractions were loaded on SDS-PAGE.

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