Figure 4.
Figure 4. EM colocalization of MHC class II molecules and GM1 in exosomal LDTI domains. (A) Daudi exosomes were lysed in 1% Triton X-100 and subjected to sucrose gradient centrifugation. Aliquots of fractions collected from the top of the gradient were analyzed by dot blot for the distribution of flotillin-1 and HLA-DRα. (B) Fraction no. 3 of the sucrose gradient was used for the EM study (upper panel). Detergent-resistant membrane isolated from Daudi cells was also analyzed for GM1 and MHC class II molecule colocalization (lower panel). Large arrows indicate gold particles (protein A gold 15) detecting anti-HLA-DRα; small arrows, gold particles (protein A gold 10) detecting bCTXB.

EM colocalization of MHC class II molecules and GM1 in exosomal LDTI domains. (A) Daudi exosomes were lysed in 1% Triton X-100 and subjected to sucrose gradient centrifugation. Aliquots of fractions collected from the top of the gradient were analyzed by dot blot for the distribution of flotillin-1 and HLA-DRα. (B) Fraction no. 3 of the sucrose gradient was used for the EM study (upper panel). Detergent-resistant membrane isolated from Daudi cells was also analyzed for GM1 and MHC class II molecule colocalization (lower panel). Large arrows indicate gold particles (protein A gold 15) detecting anti-HLA-DRα; small arrows, gold particles (protein A gold 10) detecting bCTXB.

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