American Society of Hematology

Figure 6.

$Figure 6. Partition in Triton X-114 of Lyn and HLA-DRα present in cell LDTI membrane or secreted within exosomes. Exosomes and LDTI membranes isolated from Daudi cells were extracted by Triton X-114 as described in “Materials and methods.” Aliquots (50 μL) of the aqueous and detergent phases and of the starting fractions were mixed with Laemmli buffer, loaded on SDS-PAGE, and analyzed for the presence of Lyn and HLA-DRα by Western blot.$

Partition in Triton X-114 of Lyn and HLA-DRα present in cell LDTI membrane or secreted within exosomes. Exosomes and LDTI membranes isolated from Daudi cells were extracted by Triton X-114 as described in “Materials and methods.” Aliquots (50 μL) of the aqueous and detergent phases and of the starting fractions were mixed with Laemmli buffer, loaded on SDS-PAGE, and analyzed for the presence of Lyn and HLA-DRα by Western blot.

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