Nonrandom X-chromosome inactivation of the patient. DNA was extracted from peripheral blood (A) and oral mucosal cells (B) of the family members. Exon 1 of the androgen receptor, which contains CAG repeat, was amplified as described in “Study design” with (+) or without (–) the digestion by methylation-sensitive HpaII before amplification. (A) Lane 1 and 2 indicate the patient; lane 3 and 4, her father; lane 5 and 6, her mother; and lane 7 and 8, her paternal grandmother. (B) Lane 1 and 2 indicate the patient; and lane 3 and 4, her mother.