CFU-GM mobilization, protease activities, and SDF-1 concentrations in mice mobilized by GROβ_T, G-CSF, or the combination of G-CSF plus GROβ_T. Cohorts of 3 B₆D₂F₁ mice/group/experiment were mobilized by a single injection of GROβ_T (2.5 mg/kg), a multiday regimen of G-CSF (50 μg/kg, twice a day for 4 days), or administration of GROβ_T on day 5 following the G-CSF regimen. All injections were scheduled so that all control and mobilized mice were evaluated at the same time in every experiment. Combined data from 5 to 7 experiments are shown. (A) Mean ± SEM fold increase in CFU-GM/mL blood. (B) Upper portion is a representative zymogram; lower potion, mean ± SEM fold change in MMP-9 gelatinolytic activity in plasma and marrow extracts determined by zymography. (C) Mean ± SEM fold change in marrow NE and CG activity. (D) SDF-1 protein concentration in plasma and marrow extracts determined by ELISA. (E) SCF protein concentration in plasma and marrow extracts determined by ELISA. Error bars indicate mean ± SEM.