Flow cytometric analysis. (A) Superimposed scatter plots (left panel) demonstrate discrete populations of isolated monocytes (red) and PMN neutrophils (blue) by analysis of side scatter (SSC) versus forward scatter (FSC). Histogram depicts cell surface annexin II expression by monocytes (red) and neutrophils (blue) as FL1 fluorescence (right panel). (B) Scatter plot of FL1 (FITC) versus FL2 (PE) fluorescence on human monocytes. Both isotype controls (left panel) and annexin II-FITC and CD14-PE signals (right panel) are shown. (C) Plot of CD14 positivity versus SSC (left panel). Histogram shows annexin II expression (FL1) among CD14⁺ cells (right panel).