Peripheral blood monocyte expression of annexin II. (A) Western blot analysis of cell surface expression of annexin II on human monocyte-depleted PBMCs (Non-monos) and monocytes (Monos). EGTA eluates or whole cell lysates (30 μg) were resolved on a 12.5% SDS-polyacrylamide gel and immunoblotted with anti–annexin II IgG. (B) Cell surface biotinylation of surface proteins on human monocytes. Surface biotinylated proteins were isolated by streptavidin precipitation, resolved on a 12.5% SDS-polyacrylamide gel, and immunoblotted with anti–annexin II IgG. Whole cell lysate protein was used as a positive control.