ICSBP induction of macrophage differentiation in Bcr/Abl-transformed ICSBP^-/- myeloid progenitor cells. (A) Wright-Giemsa stain of Tot2 and Tot2p210 cells transduced with the control or ICSBP retrovirus on day 6 after transduction (original magnification × 600). (B) Semiquantitative reverse transcriptase-PCR (RT-PCR) analysis for macrophage differentiation-related genes. Expression of scavenger receptor (SR) and myeloperoxidase (MPO) transcripts was analyzed using RNA samples from indicated days after transduction. β-Actin was used as a control for equal loading. (C) Induction of LPS/IFNγ-responsive genes. Cells on 6 days after transduction were treated with 200 ng/mL LPS or 200 U/mL IFNγ for 6 hours. Indicated transcripts were analyzed by semiquantitative RT-PCR. iNOS indicates inducible nitric oxide synthase; and FcγRI, Fcγ receptor I.