Immunostaining of cell lines for intracellular signaling molecules. All staining was performed on cell pellets and/or cytospins by the immunoperoxidase technique. (Top row) Left: The T-cell lymphoma line CCRF/CEM shows cytoplasmic and nuclear staining with anti-Syk antibody. Right: Scattered cells stain weakly with anti-BLNK. (Middle row) Left: Scattered cells in the Jurkat T-cell lymphoma line show weak Syk-cytoplasmic labeling. Right: Lyn membrane-associated labeling of rare cells in the Hodgkin disease cell line KMH2 (arrowed). (Bottom row) Left and center: Single cells in the L428 Hodgkin disease cell line show membrane-associated staining for Lyn (arrowed) and weak cytoplasmic labeling for Syk. Right: The periphery of a single cell in the L1236 Hodgkin disease cell line is stained for Fyn. Original magnifications: × 40 (top, middle, and left and right panels of bottom row), × 60 (bottom row, center panel).