Validation of GeneChip results by real-time RT-PCR. Experiments were performed with the use of 3 pools (pool-AA1, pool-N1, and pool-AA3): pool-AA1 and pool-N1 had been subjected to GeneChip analysis, and pool-AA3 was prepared from a fresh corhort of patients. Nine genes that showed differential expression in AA patients in the GeneChip analysis were selected: 5 were up-regulated and 4 were down-regulated. Six genes showed a consistent differential change in real-time PCR. Another 3 genes showed no changes between AA patients and healthy donors by this assay. Upward- and downward-pointing bars represent higher or lower expression levels in CD34 cells of AA patients compared with those of healthy volunteers. Black bar indicates GeneChip results; hatched bar, real-time PCR results; P1, pool-AA1; P3, pool-AA3. Mean values of 2 independent experiments in duplicate are indicated.
