Polyploidy in p21^+/+ and p21^–/– c-kit⁺, Lin^– cells expressing Survivin constructs. Transduced GFP⁺, c-kit⁺, Lin^– cells were FACS sorted and stained with 7-AAD. The proportion of c-kit⁺, Lin^– cells with more than 4 N DNA content in the viable cells, defined by forward and side scatter, was quantitated by flow cytometry and compared with p21^+/+ cells transduced with vector alone. Data are expressed as the mean ± SEM percentage increase in polyploid cells for each transduced group for 3 experiments. – indicates vector control; W, Survivin; T34A, T34A Survivin; AS, antisense Survivin.