Figure 1.
SELDI-TOF mass spectra of profiling and antibody capture experiments. (A) Weak cation exchange (WCX2) ProteinChip array. Plasma samples of a Gaucher patient before (i) and after (ii) treatment and a control experiment with recombinant CCL18 in 0.5% (wt/vol) bovine serum albumin (iii). Mass range 7000 to 8500 m/z. (B) PS20 ProteinChip array coated with monoclonal antibodies. Anti-CCL18 (iv-viii) and (as a control) anti–TNF-α (ix). Recombinant CCL18 (iv), sera of Gaucher patients (v-vi), control sera (vii-viii) and a pool of patient samples (ix) were exposed to the antibody-coated arrays. Mass range 7000 to 8500 m/z.