Figure 4.
Figure 4. Clonogenic cell survival of ATM-null cells transfected with wild-type or mutant ATM. (A) Western blot analysis showing expression of ATM protein (detected by anti–ATM antibody) in ATM-null (GM05849C) cells stably transfected with pEBS-YZ5 (wild-type ATM expression vector) or pEBS-YZ5/4949G (N1650S variant ATM expression vector). No ATM protein was detectable in mock-transfected (pEBS7) cells, and equal amounts of wild-type and variant ATM were expressed in ATM-null cells. Equivalence of loading of cell lysates is shown by anti–α-tubulin labeling. (B) Cell survival by clonogenic activity 2 weeks after various doses of X-irradiation (IR). The ATM-null cell line GM05849C was transfected with pEBS7 (mock transfectant; , pEBS-YZ5 (wild-type ATM expression vector; ⋄), or pEBS-YZ5/4949G (N1650S variant ATM expression vector; ○). Data represent mean values from 4 independent experiments.

Clonogenic cell survival ofATM-null cells transfected with wild-type or mutantATM. (A) Western blot analysis showing expression of ATM protein (detected by anti–ATM antibody) in ATM-null (GM05849C) cells stably transfected with pEBS-YZ5 (wild-type ATM expression vector) or pEBS-YZ5/4949G (N1650S variant ATM expression vector). No ATM protein was detectable in mock-transfected (pEBS7) cells, and equal amounts of wild-type and variant ATM were expressed in ATM-null cells. Equivalence of loading of cell lysates is shown by anti–α-tubulin labeling. (B) Cell survival by clonogenic activity 2 weeks after various doses of X-irradiation (IR). The ATM-null cell line GM05849C was transfected with pEBS7 (mock transfectant; , pEBS-YZ5 (wild-type ATM expression vector; ⋄), or pEBS-YZ5/4949G (N1650S variant ATM expression vector; ○). Data represent mean values from 4 independent experiments.

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